4-(gamma-L-glutamylamino)butanoyl-(BtrI acyl-carrier protein) monooxygenase (, btrO (gene)) is an enzyme with systematic name 4-(gamma-L-glutamylamino)butanoyl-(BtrI acyl-carrier protein),FMN:oxygen oxidoreductase (2-hydroxylating). This enzyme catalyses the following chemical reaction
4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2 4-(gamma-L-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O
4-(gamma-L-glutamylamino)butanoyl-(BtrI acyl-carrier protein) monooxygenase catalyses a step in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family.
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