Beta-carotene 3-hydroxylase (, beta-carotene 3,3'-monooxygenase, CrtZ) is an enzyme with systematic name beta-carotene,NADH:oxygen 3-oxidoreductase . This enzyme catalyses the following chemical reaction

beta-carotene + 2 NADH + 2 H+ + 2 O2 zeaxanthin + 2 NAD+ + 2 H2O (overall reaction) (1a) beta-carotene + NADH + H+ + O2 beta-cryptoxanthin + NAD+ + H2O (1b) beta-cryptoxanthin + NADH + H+ + O2 zeaxanthin + NAD+ + H2O

Beta-carotene 3-hydroxylase requires ferredoxin and Fe(II).

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