DSTO may refer to:
Defence Science and Technology Organisation, Australia, now Defence Science and Technology Group Defence Science and Technology Organization, Pakistan Defence Survive, Evade, Resist, Extract Training Organisation, United Kingdom Dual Stage To Orbit/Double Stage To Orbit, a spacecraft in which two distinct stages provide propulsion consecutively in order to achieve orbital velocity Divisional sea transport officer, a naval officer responsible for transport. Dwarka Sector 21 metro station, Delhi, India, by Delhi Metro station code