Stenolebias is a genus of fish in the family Rivulidae. These relatively rare annual killifish are endemic to seasonal waters in the Pantanal in Brazil.

They are small fish, up to in total length.

Species Stenolebias and the closely related Maratecoara, Papiliolebias, Pituna and Plesiolebias form a clade, Plesiolebiasini.

There are currently two recognized species in Stenolebias:

Stenolebias bellus W. J. E. M. Costa, 1995 Stenolebias damascenoi (W. J. E. M. Costa, 1991)

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