Nematolebias is a genus of fish in the family Rivulidae. These threatened annual killifish are endemic temporary waters, like pools, in the Atlantic Forest in Rio de Janeiro state, Brazil (the equally threatened killifish genera Leptolebias and Notholebias are restricted to the same state).Costa, W.J.E.M., Amorim, P.F. & Aranha, G.N. (2014): Species limits and DNA barcodes in Nematolebias, a genus of seasonal killifishes threatened with extinction from the Atlantic Forest of south-eastern Brazil, with description of a new species (Teleostei: Rivulidae). Ichthyological Exploration of Freshwaters, 24 (3) : 225-236.

They are small fish that are up to in total length. Like other killifish, the males are more colorful than females.

Species There are currently three recognized species in this genus:

Nematolebias catimbau W. J. E. M. Costa, Amorim & Aranha, 2014 Nematolebias papilliferus W. J. E. M. Costa, 2002 Nematolebias whitei G. S. Myers, 1942 (Rio pearlfish)

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