The women's 100 metre freestyle S3 event at the 2012 Paralympic Games took place on 3 September, at the London Aquatics Centre.

Two heats were held, one with five swimmers and one with six competitors. The swimmers with the eight fastest times advanced to the final.

Heats Rank Heat Lane Name Nationality Time Notes 1 2 4 Olga Sviderska 1:45.34 Q, WR 2 1 4 Xia Jiangbo 1:49.01 Q 3 2 5 Patricia Valle 2:07.22 Q 4 1 5 Yip Pin Xiu 2:09.74 Q 5 2 3 Semicha Rizaoglou 2:17.82 Q 6 1 3 Annke Conradi 2:24.12 Q 7 2 2 Haideé Aceves 2:28.16 Q 8 1 6 Iryna Sotska 2:30.85 Q 9 1 2 Vera Thamm 2:34.63 10 2 6 Ganna Ielisavetska 2:37.85 11 2 7 Maria Kalpakidou 2:54.88

Final Rank Lane Name Nationality Time Notes 5 Xia Jiangbo 1:44.32 4 Olga Sviderska 1:52.91 3 Patricia Valle 1:59.76 4 6 Yip Pin Xiu 2:09.41 5 2 Semicha Rizaoglou 2:19.92 6 7 Annke Conradi 2:21.92 7 1 Haideé Aceves 2:28.96 8 8 Iryna Sotska 2:35.66

References