Limestone Creek is a tributary of the Tennessee River in Tennessee and Alabama.

Limestone Creek may also refer to:

Streams (alphabetical by state) Limestone Creek (Chattahoochee River tributary), in Georgia Limestone Creek (Ocmulgee River tributary), a tributary of the Ocmulgee River in Georgia Limestone Creek (Kansas), a tributary of the Little Osage River in Bourbon and Allen counties Limestone Creek (Solomon River tributary), in Kansas Limestone Creek (Miami Creek tributary), in Missouri Limestone Creek (Turnback Creek tributary), in Missouri Limestone Creek (Cowaselon Creek), site of Buttermilk Falls in Madison County, New York Limestone Creek (Chittenango Creek tributary), in New York

Settlements Limestone Creek, Florida, a census-designated place in Palm Beach County