Spruce Creek may refer to:
Canada Spruce Creek (British Columbia), a stream in the Atlin Country
United States Colorado Spruce Creek (Larimer County, Colorado), a tributary of the Big Thompson River
Florida Spruce Creek Airport, in Port Orange Spruce Creek High School, in Port Orange Spruce Creek (Florida), a tributary of the Halifax River
Iowa Spruce Creek (Iowa) a tributary of the Upper Mississippi River
Maine Spruce Creek (Maine), a creek in York County, Maine
New York Spruce Creek (New York), crossed by the Salisbury Center Bridge in Herkimer County
Pennsylvania Spruce Creek (Pennsylvania), a tributary of the Little Juniata River Spruce Creek, Pennsylvania, an unincorporated community Spruce Creek Township, Pennsylvania
West Virginia Spruce Creek (West Virginia), a stream in Ritchie County