Berkhout is a Dutch surname. Notable people with the surname include:
Bernard Berkhout (born 1961), Dutch physician and jazz clarinetist Christine Marie Berkhout (1893–1932), Dutch mycologist Guus Berkhout (born 1940), Dutch engineer Jorn Berkhout (born 2002), Dutch football player Lobke Berkhout (born 1980), Dutch sailor Nina Berkhout (born 1975), Canadian novelist and poet