Guadiana may refer to:
Guadiana, a river in southern Spain and Portugal Ojos del Guadiana Guadiana Valley Natural Park, a natural park in southeastern Portugal Guadiana International Bridge Guadiana, Naranjito, Puerto Rico, a barrio of Naranjito, Puerto Rico Guadiana, Badajoz, a town and municipality in Extremadura, Spain (formerly Guadiana del Caudillo) Guadiana River (Puerto Rico), a river in Puerto Rico Guadiana Bay, a bay in western Cuba Ribera del Guadiana, a Spanish protected designation of origin (Denominación de Origen - DO) for wines in Extremadura Guadiana Trophy, an annual football (soccer) tournament hosted by Portugal and played in the pre-season