B16 or B-16 may refer to:
Transport Road B16 (New York City bus) serving Brooklyn Honda B16 engine Volvo B16 engine Bundesstraße 16, federal highway in Germany Rail & air LNER Class B16, a class of British steam locomotives Martin XB-16, a proposed bomber aircraft
Sciences Biology HLA-B16, an HLA - B serotype B16 melanoma, a cell line Electrical & mechanical 16 amp, type B – a standard circuit breaker current rating B16 Standardization of Valves, Flanges, Fittings, and Gaskets - ASME committee and specification group. Chemistry Boron-16 (B-16 or 16B), an isotope of boron
Other uses Pope Benedict XVI One of the ECO codes for the Caro–Kann Defence in chess