Sam Querrey defeated John Isner in the final, 3–6, 7–6(7–4), 6–4 to win the singles tennis title at the 2010 Serbia Open.
Novak Djokovic was the defending champion, but retired in the quarterfinals against Filip Krajinović.
Seeds The first four seeds gets a first-round bye.
Draw
Finals
Top half
Bottom half
References Main Draw Qualifying Singles