Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition Ingredients (g/L)Middlebrook 7H10 Agar Base Ammonium sulfate, 0.50 Monopotassium phosphate, 1.50 Disodium phosphate, 1.50 Sodium citrate, 0.40 Magnesium sulfate, 0.025 Zinc sulfate, 0.001 Copper sulfate, 0.001 L-Glutamic acid, 0.50 Ferric ammonium citrate, 0.04 Pyridoxine hydrochloride, 0.001 Biotin, 0.0005 Malachite green, 0.00025 Agar, 15.00 Calcium chloride, 0.0005
Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
See also Lowenstein-Jensen medium Middlebrook 7H9 Broth
References