Charles Best may refer to:
Charles Best (poet) (1570–1627), English poet Charles Best (British Army officer) (1765–1836), British army officer Charles Best (American football) (1874–1962), American college football and basketball coach Charles Best (medical scientist) (1899–1978), American-Canadian medical scientist and co-discoverer of insulin Charles Best (politician) (1909–1996), Australian politician Charles Best (businessman), American businessman and founder of Donorschoose.org Charles Alexander Best (1931–1978), Canadian politician, son of Charles Herbert Best