Martina Hingis was the defending champion but did not compete that year.
Venus Williams won in the final 6–3, 6–0 against Meghann Shaughnessy.
Seeds A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. The top four seeds received a bye to the second round.
Venus Williams (champion) Amanda Coetzer (semifinals) Conchita Martínez (second round) Arantxa Sánchez-Vicario (quarterfinals) Magdalena Maleeva (second round) Justine Henin (quarterfinals) Meghann Shaughnessy (final) Magüi Serna (first round)
Draw
Final
Top half
Bottom half
External links 2001 Betty Barclay Cup draw