Patricia Wartusch was the defending champion but lost in the second round to Mariana Díaz Oliva.
Paola Suárez won in the final 6–4, 6–2 against Rita Kuti-Kis.
Seeds A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. The top two seeds received a bye to the second round.
Paola Suárez (champion) Corina Morariu (second round) Ángeles Montolio (second round) Tathiana Garbin (first round) Marta Marrero (first round) Rita Kuti-Kis (final) Sylvia Plischke (first round) Anabel Medina Garrigues (first round)
Draw
Final
Section 1
Section 2
External links 2001 Copa Colsanitas Draw