Patricia Wartusch was the defending champion but lost in the second round to Mariana Díaz Oliva.

Paola Suárez won in the final 6–4, 6–2 against Rita Kuti-Kis.

Seeds A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. The top two seeds received a bye to the second round.

Paola Suárez (champion) Corina Morariu (second round) Ángeles Montolio (second round) Tathiana Garbin (first round) Marta Marrero (first round) Rita Kuti-Kis (final) Sylvia Plischke (first round) Anabel Medina Garrigues (first round)

Draw

Final

Section 1

Section 2

External links 2001 Copa Colsanitas Draw