Golden thread may refer to:

Biology Nemipterus virgatus, or golden threadfin bream, a species of marine ray-finned fish Cuscuta pacifica, or goldenthread, a species of dodder plant Coptis trifolia, or threeleaf goldenthread, a circumboreal plant in the Buttercup family

Law Golden thread (law), a legal judgment iterating the duty incumbent on the prosecution to prove the accused's guilt beyond a reasonable doubt

Literature The Golden Thread (novel), the second book of the novel A Tale of Two Cities by Charles Dickens The Golden Thread: A History of the Western Tradition, a 2025 history book series by Allen C. Guelzo and James Hankins The Golden Thread: Esoteric Hitlerism, a 1978 book by Miguel Serrano The Golden Thread, a 1989 novel by Suzy McKee Charnas

See also Gold thread