Olot District () is a district of Bukhara Region in Uzbekistan. The capital lies at the city Olot. It has an area of and its population is 101,300 (2021).Urban and rural population by region, Bukhara regional department of statistics .

The district consists of 1 city (Olot), 8 urban-type settlements (Ganchi Chandir, Kesakli, Qirtay, Sola qorovul, Jayxunobod, Oʻzbekiston, Chovdur, Boʻribek Chandir) and 10 rural communities.

The etymology of the name comes from the Turkic tribe Alat or Ala-at, also known in Arabic and Persian as Khalaj, and in Chinese as Boma, Hela, and Heloγ, all with a meaning "piebald horse". During the Middle Age, Alats played a prominent role in the history of southern Uzbekistan, Khorasan (Kalat), Persia and Afghanistan (Khalaj).

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