Valley Vipers may refer to:
Science: Montivipera latifii, a venomous viper species endemic in Iran
Sports: Rio Grande Valley Vipers, a team in the NBA Development League Chattahoochee Valley Vipers, a former team in the American Indoor Football Association Roanoke Valley Vipers, a former team in the Southern Professional Hockey League Tennessee Valley Vipers, a professional arena football team in the now-defunct AF2 development league Valley Vipers, a minor league team based in Scottsdale, Arizona, that played in the Western Baseball League in 2000