B35 may refer to:
Aircraft Beechcraft Model 35 Bonanza, 1950 version Northrop YB-35, an experimental aircraft Avia B-35, a Czechoslovak aircraft
Roads Bundesstraße 35, a German road B-35 (Michigan county highway), United States B35 road in County Tyrone, Northern Ireland; see List of B roads in Northern Ireland
Other B35 (New York City bus) in Brooklyn HLA-B35, an HLA-B serotype