65th Regiment may refer to:

65th (2nd Yorkshire, North Riding) Regiment of Foot, a unit of the British Army 65th Infantry Regiment (Philippines) 65th Infantry Regiment (United States), a unit of the United States Army 65th Air Defense Artillery Regiment, a unit of the United States Army 65th Armoured Regiment (India), an armoured unit of the Indian Army

American Civil War regiments 65th Indiana Infantry Regiment, Union Army 65th Illinois Volunteer Infantry Regiment, Union Army 65th New York Volunteer Infantry, Union Army 65th Ohio Infantry, Union Army

See also 65th Division (disambiguation) 65th Squadron (disambiguation)