James Holmes may refer to:
Artists Jimmy "Duck" Holmes (born 1947), American blues musician James Flournoy Holmes, album cover artist James Holmes (actor) (born 1965), British actor James Holmes (artist) (1777–1860), English painter James Roland Holmes (1939–1999), organist, partner of Ned Rorem James S. Holmes (1924–1986), Dutch poet and translator
Criminals James Holmes (mass murderer) (born 1987), convicted of the 2012 Aurora theater shooting James William Holmes (1956–1994), convicted murderer of Don Lehman
Public servants James Holmes (politician) (1831–1910), member of the New Zealand Legislative Council James Howard Holmes (born 1943), diplomat James Leon Holmes (born 1951), U.S. federal judge James M. Holmes (born 1957), United States Air Force general
Sportspeople Ducky Holmes (James William Holmes, 1869–1932), baseball player Jim Holmes (baseball) (1882–1960), baseball pitcher Jimmy Holmes (footballer, born 1908) (1908–1971), English footballer Jimmy Holmes (footballer, born 1953), Irish footballer Jim Holmes (footballer) (born 1954), Scottish footballer
Trade unionists James Holmes (trade unionist) (1861–1934), English railway industry trade unionist Jimmy Holmes (trade unionist) (1850–1911), English hosiery industry trade unionist
Other James H. Holmes (1826–1900), Baptist minister in Richmond, Virginia
See also Jamie Holmes (disambiguation)