Ishino () is a Japanese surname. Notable people with the surname include:
, Japanese speed skater Mako Ishino (born 1961), Japanese singer and actress Mami Ishino (born 1983), Japanese hurdler , Japanese handball player Ryuzou Ishino (born 1962), Japanese voice actor Takkyu Ishino (born 1967), Japanese composer and music producer Yōko Ishino (born 1968), Japanese actress Yoshizumi Ishino (born 1959), Japanese molecular biologist, known for his discovering the DNA sequence of CRISPR
See also Marin Ishino, alias of Yoshinori Sunahara, Japanese DJ Ishino Station, railway station in Miki, Japan Shimo-Ishino Station, abandoned railway station in Miki, Japan