Pantothenase () is an enzyme that catalyzes the chemical reaction

The enzyme characterised from Pseudomonas bacteria hydrolyses vitamin B5, pantothenic acid, to (R)-pantoic acid and β-alanine.

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (R)-pantothenate amidohydrolase. Other names in use include pantothenate hydrolase, and pantothenate amidohydrolase.

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