Glutaryl-7-aminocephalosporanic-acid acylase () is an enzyme characterised from a Pseudomonas species that catalyzes the hydrolysis of the cephalosporin antibiotic, glutaryl-7-aminocephalosporanic acid, to the parent 7-ACA and glutaric acid:
The structure of several of these enzymes have been determined. An immobilised version of the enzyme has been produced as a means of making 7-ACA for the manufacture of other derivatives.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase. Other names in use include 7beta-(4-carboxybutanamido)cephalosporanic acid acylase, cephalosporin C acylase, glutaryl-7-ACA acylase, CA, GCA, GA, cephalosporin acylase, glutaryl-7-aminocephalosporanic acid acylase, and GL-7-ACA acylase.
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