5-oxoprolinase (ATP-hydrolysing) () is an enzyme characterised from rat kidney that catalyzes the hydrolysis reaction:
The enzyme uses adenosine triphosphate to drive the reversible reaction towards L-glutamic acid: its byproducts are adenosine diphosphate and orthophosphate. The reaction is part of the glutathione cycle.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-oxo-L-proline amidohydrolase (ATP-hydrolysing). Other names in common use include pyroglutamase (ATP-hydrolysing), oxoprolinase, pyroglutamase, 5-oxoprolinase, pyroglutamate hydrolase, pyroglutamic hydrolase, L-pyroglutamate hydrolase, 5-oxo-L-prolinase, and pyroglutamase.
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