Prunasin β-glucosidase () is an enzyme characterised from Prunus serotina that catalyzes the chemical reaction:
The cyanogenic glycoside, prunasin, is produced from amygdalin by a similar hydrolysis reaction catalysed by amygdalin beta-glucosidase. Bacteria can produce hydrogen cyanide as a defense mechanism via these pathways.
The enzyme is a hydrolase, specifically a glycosidase that hydrolyses O- and S-glycosyl compounds. The systematic name is prunasin β-D-glucohydrolase. It is also called prunasin hydrolase.
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