Route 241 or Highway 241 may refer to:
Canada Manitoba Provincial Road 241 Prince Edward Island Route 241 Quebec Route 241
Costa Rica National Route 241
Cuba
Pinar del Rio–Vinales Road (1–241) Cotorro–Jaruco Road (2–241)
Japan Japan National Route 241
United Kingdom B241 road
United States U.S. Route 241 (former) U.S. Route 241 (1926) U.S. Route 241 (1930s) Alabama State Route 241 Arkansas Highway 241 California State Route 241 Florida State Road 241 (former) Georgia State Route 241 Indiana State Road 241 Kentucky Route 241 Maryland Route 241 (former) Minnesota State Highway 241 Montana Secondary Highway 241 New Mexico State Road 241 New York State Route 241 North Carolina Highway 241 Ohio State Route 241 Oregon Route 241 Pennsylvania Route 241 Tennessee State Route 241 Texas State Highway 241 (former) Texas State Highway Spur 241 Farm to Market Road 241 Utah State Route 241 Virginia State Route 241 Washington State Route 241 Wisconsin Highway 241 Wyoming Highway 241