Holsinger (in Germany mostly spelled Holzinger) is a German surname. Notable people with the surname include:

David Holsinger (born 1945), American composer and conductor Henry R. Holsinger (1833–1905), American pastor, publisher and leader of The Brethren Church James W. Holsinger (born 1939), American physician and retired major general of the U.S. Army nominated to be Surgeon General of the United States Rufus W. Holsinger (1866–1930), American photographer

See also Holzinger (disambiguation)