Ghirlandaio () is the surname of a family of Renaissance Italian painters:
Domenico Ghirlandaio (1449–1494), painter of fresco cycles and Michelangelo's teacher Davide Ghirlandaio (1452–1525), younger brother of Domenico Benedetto Ghirlandaio (1458–1497), younger brother of Domenico and Davide Ridolfo Ghirlandaio (1483–1561), son of Domenico
See also Michele Tosini (1503–1577), Italian painter, also known as Michele del Ghirlandaio, apprentice of Ridolfo