Ambrosini is an Italian surname. Notable people with the surname include:
Abele Ambrosini (1915–1943), Italian partisan Bartolomeo Ambrosini (1588–1657), Italian botanist, physician and naturalist Brenno Ambrosini, Italian pianist Carlo Ambrosini (1954–2023), Italian comic book artist and writer Cesare Ambrosini (born 1990), Italian footballer Chiara Ambrosini (born 2006), Argentine field hockey player Claudio Ambrosini (born 1948), Italian composer and conductor Dario Ambrosini (1918–1951), Italian Grand Prix motorcycle road racer Emilio Ambrosini (1850–1912), Italian architect Enrique Domingo Dussel Ambrosini (1934–2023), Argentine theologian and historian Ernesto Ambrosini (1894–1951), Italian athlete who competed mainly in the 3000 metres steeplechase Filippo Ambrosini (born 1993), Italian pair skater Floriano Ambrosini (1557–1621), Italian architect and engineer Gaspare Ambrosini, (1886–1986), Italian statesman James Ambrosini (born 1991), Australian-born Italian rugby union player Marco Ambrosini, (born 1964), Italian composer and musician living in Germany Maria Luisa Ambrosini, Italian author Mario Oriani-Ambrosini (1960–2014), Italian constitutional lawyer and politician Massimo Ambrosini, (born 1977), Italian footballer Rachelina Ambrosini (1925–1941), Italian teenager who is venerated by the Roman Catholic Church Tito Ambrosini (1903–1965), Italian water polo player Vittorio Ambrosini (1893–1971), Italian politician