S65 may refer to:
Automobiles BMW S65, an automobile engine Daihatsu Hijet (S65), a Japanese kei truck Mercedes-Benz S65 AMG, a sedan S-65 Stalinets, a Soviet tractor
Rail S65 (Esko Prague), a commuter rail line in the Czech Republic S65, a Schaffhausen S-Bahn railway service in Switzerland and Germany
Other uses Darkinjung language
, a submarine of the Indian Navy Savoia-Marchetti S.65, an Italian racing seaplane Siemens S65, a mobile phone Sikorsky S-65, a family of heavy-lift transport helicopters S65, a postcode district in Rotherham, England