The following highways are numbered 401:
Canada British Columbia Highway 401 (former) Manitoba Provincial Road 401 Ontario Highway 401
Costa Rica National Route 401
Cuba
Road to El Fe (4–401)
Japan Japan National Route 401
Norway Norwegian County Road 401
Thailand Thailand Route 401
United States U.S. Route 401 Florida: Florida State Road 401 County Road 401 (Brevard County, Florida) Georgia State Route 401 (unsigned designation for Interstate 75) Kentucky Route 401 Louisiana Highway 401 Nevada State Route 401 New York: New York State Route 401 (former) County Route 401 (Albany County, New York) North Carolina Highway 401 (former) Pennsylvania Route 401 Pennsylvania Route 401 Alternate Truck Puerto Rico Highway 401 Rhode Island Route 401 Virginia State Route 401 Washington State Route 401