Catechol 2,3-dioxygenase (, 2,3-pyrocatechase, catechol 2,3-oxygenase, catechol oxygenase, metapyrocatechase, pyrocatechol 2,3-dioxygenase) is an enzyme with systematic name catechol:oxygen 2,3-oxidoreductase (decyclizing). This enzyme catalyses the following chemical reaction
This enzyme contains Fe(II).
The substrate spectrum includes Catechol + O2, 3-Methylcatechol + O2, 4-Methylcatechol + O2, 4-Fluorocatechol + O2, 3-Chlorocatechol + O2, 3,4-Dihydroxybenzoate + O2, Pyrogallol + O2, and Protocatechualdehyde + O2; inhibitors include Monoiodoacetate (weak), o-Phenanthroline (weak), 8-Hydroxyquinoline (weak), Fe2+, 3-[(Methylthio)methyl]catechol, 3-Chlorocatechol, 4-Chlorocatechol, Tiron, CuCl, CuSO4, o-Nitrophenol, m-Phenanthroline, ATP, Mg2+, Na2B4O7 (i.e. Borax), p-Chloromercuribenzoate, Acetone, Hg2+, Ag+, and NO; Metal compounds/salts include Fe2+ (4 atoms of iron per mol of enzyme, 1 atom of iron per molecule, 3 atoms of irons per mol of enzyme, active site Fe2+, activity is closely related to specific content of iron in this protein, FeCl2 and FeSO4 activate metapyrocatechase 2, no effect on metapyrocatechase 1.
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